Research Services

Research Services

(scroll down for service price list)

Project set up and design:

• Pre-analysis consulting. Consulting services include analysis of project set up/design, overall goals of the project, protocol review, discussion of appropriate sample types, collection techniques, genes of interest, and relevant housekeeping (reference) gene(s).

• Sample preparation. The Core Facility offers assistance in sample collection/preparation. Established protocols and sample stabilization solution are available upon request.

• Reference gene validation. It is important to pick a stable reference gene when doing gene expression analysis to ensure unbiased results. A reference gene validation experiment is run prior to profiling in order to determine the most stably transcribed reference gene.

• Materials and methods. The Core Facility can provide a materials and methods write up for publication upon request.

Nucleic acid extraction/cDNA synthesis:

• NA extraction available for nearly all species and sample types.

• Semi-automated, high-throughput extraction. gDNA and total RNA extractions for research projects are performed on the Qiagen QIAcube HT, a 96-well filter-based extraction system from Qiagen.

• cDNA synthesis. Complimentary DNA (cDNA) can be synthesized from extracted RNA upon request.

qPCR:

• Assays. The Core Facility has many qPCR systems in-house for numerous species including chicken, canine, equine, feline, human, mouse, non-human primate, rat, and trout. These assays may also be purchased for external use. Please visit our website for a full list of available systems.

• Custom qPCR assay design. If the assay you need is not offered by the Core Facility, but the gene of interest has been sequenced, then the Core Facility can provide custom primer/probe design utilizing Primer Express 3 software. The facility offers full disclosure of primer and probe sequences for these assays.

• SNP. SNP genotyping analysis identifies single nucleotide polymorphisms which are responsible for differences in genetic traits, susceptibility to disease or response to drug therapies. Over 4.5 million human and 10 thousand mouse SNPs are ready to order assays from Applied Biosystems.

qPCR analysis:

• qPCR thermocycler. All qPCR analysis is performed using Applied Biosystems QuantStudio 5 or 7 Pro Real-time PCR Systems. The Core Facility also offers the use of the thermocyclers to external clients who bring in their own pipetted and barcoded 384-well plates.

• Final result calculations. Results can be reported by raw Cq values, using absolute standard methods, or the comparative Cq method for relative gene transcription (i.e. Livak or Pfaffl Methods).

Price List:

**must have enough samples to purchase kit